PVX syn-tasiRNA precursor designer

Standalone mini web app to build the dsDNA insert for PVX-based syn-tasiRNA constructs.

Input: precursor name, one 22-nt miRNA target site, and one or more 21-nt syn-tasiRNAs.

1. Input

Precursor name

22-nt miRNA target site

Module sequence

Full dsDNA insert (5'→3')

Forward oligo / top strand (5'→3')

Reverse oligo / bottom strand (5'→3')