P-SAMS
The Plant Small RNA Maker Suite (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. Use the webtool here, or download the script here if you prefer the command line.
If using P-SAMS please cite:
Fahlgren, N, Hill ST, Carrington JC, Carbonell A (2016) P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design. Bioinformatics 32: 157-158.
Art-sRNA B/c vectors
We have developed several plant BsaI/ccdB-based (“B/c”) vectors for high-throughput cloning of amiRNAs and syn-tasiRNAs. All vectors should be requested at addgene.org. Find the cloning protocol here.
| AmiRNA vector | Plant species | Publication | Addgene number |
| pENTR-AtMIR390a-B/c | – | Carbonell Plant Phys 2014 | 51778 |
| pFK210B-AtMIR390a-B/c | Eudicots | Carbonell Plant Phys 2014 | 51777 |
| pMDC123SB-AtMIR390a-B/c | Eudicots | Carbonell Plant Phys 2014 | 51775 |
| pMDC32B-AtMIR390a-B/c | Eudicots | Carbonell Plant Phys 2014 | 51776 |
| pENTR-BS-AtMIR390a-B/c | Eudicots | Cisneros NAR 2023 | 199559 |
| pMDC32B-BS-AtMIR390a-B/c | Eudicots | Cisneros NAR 2023 | 199560 |
| pENTR-OsMIR390-B/c | – | Carbonell Plant J 2015 | 61468 |
| pMDC123SB-OsMIR390-B/c | Monocots | Carbonell Plant J 2015 | 61466 |
| pMDC32B-OsMIR390-B/c | Monocots | Carbonell Plant J 2015 | 61467 |
| pH7WG2B-OsMIR390-B/c | Monocots | Carbonell Plant J 2015 | 61465 |
| Syn-tasiRNA Vector | Plant species | Publication | Addgene number2 |
| pENTR-AtTAS1c-B/c | – | Carbonell Plant Phys 2014 | 51774 |
| pMDC123SB-AtTAS1c-B/c | A. thaliana1 | Carbonell Plant Phys 2014 | 51772 |
| pMDC32B-AtTAS1c-B/c | A. thaliana | Carbonell Plant Phys 2014 | 51773 |
| pENTR-AtTAS1c-D2-B/c | – | López-Dolz NAR 2020 | 137883 |
| pMDC32B-AtTAS1c-D2-B/c | A. thaliana | López-Dolz NAR 2020 | 137884 |
| pMDC32B-AtTAS1c-D2-B/c-AtMIR173 | All | López-Dolz NAR 2020 | 137885 |
1MiR173 is a non-conserved miRNA, thus a construct expressing miR173 has to be co-expressed with the syn-tasiRNA construct to induce syn-tasiRNA biogenesis in non-Arabidopsis species